Gas chromatograph coupled to an isotope ratio mass spectrometer

Contacts :

Technical managers : Rachel Boscardin et Claude Le Milbeau
Scientific correspondent : Claude Le Milbeau

Brand and model :

Thermo Scientific Trace GC Ultra, coupled to an AS3000 autosampler, a GC ISOLINK (Thermo), a CONFLO IV (Thermo) and a Thermo Scientific Delta V Advantage isotope ratio mass spectrometer.

Principle :

Separation of a lipid fraction by GC, conversion of organic molecules into gases (CO/CO2/H2) and analysis of these gases by isotope ratio mass spectrometry.

Objectives :

Determination of the H (δ2H), C (δ13C) or O (δ18O) isotopic composition of molecular biomarkers.

Samples processed :

lipid extracts, sediments, rocks, plants.

Pretreatments :

Lipid extraction by ASE200, Separation of total lipid extracts into chemical fractions by Aspec GX271.

Results :

Chromatogram and isotopic compositions (δ2H, δ13C and δ18O) of molecular biomarkers